This book covers important aspects of modern optical microscopy and image restoration technologies. Instead of pure optical treatment, the book is delivered with the consideration of the scientists who utilize optical microscopy in their daily research. However, enough details are provided in basic imaging principles, optics and instrumentation in microscopy, spherical aberrations, deconvolution and image restoration. A number of microscopic technologies such as polarization, confocal and multi-photon microscopy are highlighted with their applications in biological and materials sciences/engineering.

Contents:

• Basic Principles of Imaging (T J Holmes & P C Cheng)
• Optics for the Biologist: What You Need to Know to Make Your Light Microscope Work Properly (J Pawley)
• Spherical Aberration in Confocal Microscopy (C J R Sheppard)
• Deconvolution and Image Quality Control � Valuable Tools in Multi-Dimensional Light Microscopy (L Schaefer & A Kriete)
• Quantitative Polarised Light Microscopy (T Wilson et al.)
• Multi-Photon Scanning Microscopy Using a Femtosecond Cr:forsterite Laser (C-K Sun et al.)
• Design and Performance Evaluation of a Multi-Functional Microscopy (W-J Chen et al.)
• Zeeman Laser Scanning Confocal Microscopy in Turbid Media (C Chou et al.)
• A Method for Real-Time Confocal Imaging of Substrate Surfaces During Active Air Abrasion Cutting: The Cutting Edge of Air Abrasion (R J Cook et al.)
• Three-Dimensional Optical Data Storage with a Multi-Layered Recording Medium (Y Kawata)
• The Studies of Single Luminescent Conjugated Polymers (S-C Yang et al.)
• Confocal Microscopy of GFP-Like Pigments in Corals (G Cox & A Salih)
• Three-Dimensional Localisation of Fluorescence Resonance Energy Transfer in Living Cells Under Two-Photon Excitation (M Gu)
• Multi-Dimensional Imaging of Cell- and Tissue-Engineered Constructs (Y J Zhu et al.)

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